Discussion question 1

Discussion question 1

The study of international business is fine if you are going to work in a large multinational enterprise, but it has no relevance for individuals who are going to work in small firms.”

This topic can be one that can be argued both ways. Technology has had a tremendous impact on how companies do business. An organization with a web site is able to connect with people all across the world. Understanding this, a company should take the time to both invest and learn advances in technology. This is not solely for large corporations, since if a small business doesn’t take advantage of these strategies, they will be left out of the competition. The days of the paperback phone book are gone. Without information technology, most businesses wouldn’t even exist.

Information technology is infiltrating all aspects of business, both large and small. Without it, a company will not be able to keep up with their competition. On the flip side of this theory however is the fact that information technology is cheap, therefore everyone had access to it. This in turn allows all the competition to have the same edge. This may work for a smaller company that has a good marketing campaign. Larger companies have always been able to succeed in the competitive market due to their ability to gain a foothold in the stock market and obtain funding that a smaller company may not be able to do. The ability to advertise allows these smaller organizations to become more lucrative simply by being recognized. A retail company that is willing to ship their products globally is in a position to increase their sales exponentially.

These smaller companies may also expand once they are recognized as a company that is able to maintain a good service or product across the country or across the world. Rather than shipping their product, they may choose to employ people from a country that buys heir goods or services, thereby increasing availability and allowing those from the host country to be a part of the company. Certain countries, such as India, may provide services at a much lower cost than in the United States, thereby creating a greater profit for the company conducting the business. Starbucks is a prime example of this type of business. Twenty-five years ago, Starbucks was hardly known. They had a small number of stores in the United States. They are now one of the most popular brands in the world, with almost 17,000 stores in 50 countries (Hill, 2012).

Vizio also is a success story for small business. They are a company that produces flat screen televisions, are known worldwide and have less than 100 employees. They outsource most of their engineering, all manufacturing and a great deal of logistics. Because it is able to outsource so much at reduced rates, the company is able to sell at discount retailers. They are also able to sell in volume. This gives the company a competitive edge against other retailers with more overhead (Hill, 2012).

With all of these things taken into consideration, it would appear that small business could benefit from globalization if properly managed.

Geraldine

Discussion 2

Prior to taking this class, I would probably have said a similar statement. It can be common for a common person to think of large corporations such as Walmart, Coca-Cola, or Samsung when the term international business is used. However, globalization is becoming more of the normal course of business as we progress through time and technology improves.

A portion of doing business internationally is exporting goods. In the United States, it is not the large companies that do most of the exporting. In fact, about 90 percent of the firms that export goods are small businesses that employ less than 100 people (Hill, 2013). Firms with less than 20 employees represent the fastest growing segment of U.S. exporting companies (Hannaher, 2010). It turns out exporting is a good fit for companies of all sizes. It makes sense, once one begins to explore the benefits of selling goods to international customers. An initial analysis can put in plain view that a company’s number of potential customers will increase dramatically once the decision is made to export. A company can go from the population of the US, to a global population. Another factor is companies can gain a market advantage by selling in a country with less competition than in the United States (Hannaher, 2010). This is especially true in developing countries or countries that are transitioning from a government controlled economy to a market driven economy (Hill, 2013). In those countries, individuals are newly able to make choices about the products they consume. Since competition had been closely regulated, there are fewer existing businesses in place. Companies that are among the first to arrive in a new market segment can enjoy a first mover advantage (Brickley, Smith, & Zimmerman, 2009). Companies can benefit by being the first to build infrastructure and be the first company that people think about when they think about their product.

Another factor that international business is for just about every business is the evolution of the internet. The internet has made international commerce extremely easy and inexpensive (Hill, 2013). Companies can build a website, and be open for business in any country in the world. It is also much more convenient to ship and transport products. In the early 1900s products were transported using trains and ships. It could take several days or weeks to ship a product from Dallas, Texas to Paris, France. Now, packages can travel to just about any destination due to air travel and overnight delivery service.

This point came to mind as I was attending a graduation party this past weekend at an old train station. Inside the train station had a map of the state of Michigan in 1910. What was striking, was the map did not have any highways! The map illustrated many train routes across the state. It was still amazing to think about that 100 years ago the only way people moved around was by train. Therefore, trips had to be well thought out. You lived and worked in your hometown, and that was about it. There was no getting in the car and travel 3 hours to a summer cottage every weekend, and there was not even travel 10 miles away because we happen to like that Costco instead of the local grocery! The same concept is true in international commerce. What was once out of reach, is now much closer, and with improving technology will surely increase international trade.

seigle

OS 3525, Legal Aspects of Safety and Health Unit I to Unit VIII Assignment

OS 3525, Legal Aspects of Safety and Health Unit I to Unit VIII Assignment

Unit I Assignment

Hi Jerry! This is your OSHA’s Area Director.

We received a non-formal complaint recently from one of your current employees. The complaint alleges that employees there are performing welding operations on stainless steel in an area with inadequate ventilation.

This employee also states that they frequently “cough up” black sputum, and that they are worried about the health consequences caused by this lack of ventilation.

The complaint further states that no air sampling has been performed to evaluate the employees’ exposures to welding fumes. And, that when asked for respirators, they were given “dust masks” with one strap, which were purchased at the local hardware store. The effectiveness of these dust masks for the exposure that is present is also in question.

I will also be sending you an email summarizing this non-formal complaint, so that you can respond accordingly.

Thanks Jerry! Goodbye.

Part 1

Draft a letter in response to the complaint. Your letter should summarize why you believe the complaint is invalid and no on-site inspection is required. Keep in mind, simply stating that the complaint is invalid is not adequate. You must support your opinions in the letter.

 

 

Part 2

(Answer the following question on the page 2 of your word document) Answer the questions below in a few sentences.

1. Where does this type of complaint fit on OSHA’s Priority criteria?

2. What factors could have made this complaint non-formal rather than formal?

3. What steps could you take as the employer to identify the employee who filed the complaint? 4. What factors could result in this complaint being reclassified as a formal complaint?

Save both parts of this assignment in one word document to submit for grading.

Unit II Assignment

Discussion Question

Question 1

Under what conditions can an employee be denied access to the opening conference, walk-around, and closing conference? Your response should be at least 75 words in length.

Question 2

If the OSHA compliance officer requests documents that are not related to a formal complaint, what options do you believe the employer has? Your response should be at least 75 words in length.

Question 3

If OSHA determines that an employer’s response to a non-formal complaint is adequate, what options does the employee filing the non-formal complaint have? Your response should be at least 75 words in length.

Question 4

Can an employee request that an attorney or union representative attend a private conference with the compliance officer? If the union demands to have a representative present, does the employee have to comply? Your response should be at least 75 words in length.

Unit II Assignment

You receive a follow-up call from the area director saying the employee filing the original non-formal complaint has provided additional information about the alleged health situation and submitted a formal complaint using the OSHA-7 form, making the complaint a formal complaint. A few days later, an OSHA compliance officer shows up at your facility to perform a comprehensive inspection. The compliance officer presents the proper credentials, and you verify that the compliance officer is employed by OSHA and assigned to the local office.

During the opening conference, the compliance officer provides you with the formal complaint, alleging that employees are exposed to hazardous concentrations of metal fumes in welding areas of the plant, that you have not performed any air sampling to determine exposure levels, that adequate ventilation is not present in welding areas, and that adequate respiratory protection has not been provided to welders. As a part of the inspection, the compliance officer requests the following documents:

 

 Chemical inventory list;

 

 OSHA 300 logs;

 

 Hazard Communication Program, including training records;

 

 any sampling data that you have;

 

 Respiratory Protection Program, including medical clearance letters and training records;

 

 written hazard assessment for personal protective equipment (PPE) used at the facility;

 

 Safety Data Sheets (SDSs) for the metals you use in the production process and any welding rods/wire used in the welding area; and

 

 any other written programs you have that are required by an OSHA regulation.

 

The compliance officer takes a walk-through tour of the facility, spending extra time in the welding areas. During the walk-through, the compliance officer points out several issues believed to be apparent violations. The issues are as follows:

 

 Heavy haze is present in the welding area.

 

 Individuals wearing half-mask air-purifying respirators have full beards.

 

 Employees are using chemicals that could be injurious to the eyes, and no emergency eyewash is present.

 

 Eyewash is present in another area of the plant that is covered in dust, and there is no indication of recent operation or inspection.

 

 Employees are using chemicals that could be absorbed through the skin and are not using any gloves.

 

 Employees are performing maintenance inside a press with no lock-out/tag-out applied.

 

 No written lock-out/tag-out program is available at the time of the inspection.

 

 Welding operation is performed near flammable materials, and no fire watch present.

 

 There is no record of training for fork truck drivers.

 

 Extension cords are stretched across walkways.

 

 Three containers are present in the plant with no label present on any of the containers.

 

 An employee could not find a SDS for the chemical he or she was using.

 

The compliance officer asks for a private conference room and a list of non-managerial employees. He tells you that he intends to interview four non-managerial employees before leaving for the day. He also states that he will return the next day to collect some air samples at the facility.

You are worried about the number of citations and penalties that you may face. Provide a document summarizing the steps you would take as soon as the compliance officer leaves, and the steps you believe you could have taken during the walk-through that may have resulted in a quick-fix penalty reduction.

Your document must be at least three pages in length, not counting the title or reference pages. You must also include at least one reference using appropriate APA style.

Information about accessing the Blackboard Grading Rubric for this assignment is provided below.

Unit III Assignment

You receive a document (linked below) by certified mail. After reading the document, prepare a response that summarizes the approach you would take to the citations and penalties that have been proposed. Be sure to include the following in your response:

 Steps you are required to take,

 Options available to you,

 Contacts you would make, and

 Documentation necessary to respond to the citations and penalties.

Your response must be a minimum of two pages in length, using at least one reference. All sources must be cited and a reference provide using APA style.  Click here to access the OSHA citation document for this assignment. Information about accessing the Blackboard Grading Rubric for this assignment is provided below.

Unit IV Assignment

Based on the Citation and Notification of Penalty letter you received in Unit III, prepare a document that summarizes at least five actions you would take as soon as possible after you reviewed the letter. For each of the actions, you must state the following:

 

 the exact action,

 

 why you believe the action is required for the citations and penalties,

 

 how you believe the actions will assist in responding to the citations and penalties, and

 

 resources you would use to accomplish the action.

 

You must support your actions with reliable sources. Your response must be a minimum of two pages in length, using at least one reference. All sources must be cited, and a reference must be provided using APA style.

Information about accessing the Blackboard Grading Rubric for this assignment is provided below.

Unit V Assignment

You managed to schedule an informal conference with the area director at the regional OSHA office four days after you receive the Notice of Citations and Penalty. Based on the citations and penalties you received in Unit III, prepare a document that lists the citations and penalties you wish to discuss with the area director.

You should summarize what you are trying to accomplish in regard to each citation/penalty you choose, to include:

 The information you will use to try and accomplish your goal,

 The information you will take with you to the meeting, and

 Who will accompany you to the meeting.

You must support your actions with reliable sources. Your grade will be based on your ability to present a case to your professor (serving as the area director) to reduce either the severity of some citations, or the amount of some penalties. If you simply state that you accept the citations and penalties as written, you will receive a minimal score on the assignment.

Your response must be a minimum of two pages in length, not including the title page and reference page. You must use at least one reference in the paper. All sources must be cited in the text and on the reference page, using APA style.

 

Unit VI Assignment

You fail to reach an informal settlement agreement with the area director. You file a Notice to Contest within the required 15-day period. Your case is assigned to an administrative law judge (ALJ). Prepare a document summarizing the case you will submit to the ALJ. The document should discuss the following at a minimum:

 Which citations and penalties you would contest,

 the reasoning behind each contested citation and/or penalty,

 Documents you would bring to the hearing,

 Individuals you would use at the hearing,

 How the case before the ALJ differs from the informal conference,

 What information will be presented before the ALJ that was not presented in the informal conference, and

 What information you would request from OSHA as part of discovery.

You must support your actions with reliable sources. Your grade will be based on your ability to present a case to your professor, serving as the ALJ, to reduce or vacate either the severity of some citations or the amount of some penalties. If you simply state that you accept the citations and penalties as written, you will receive a minimal score on the assignment.

Your response must be a minimum of two pages in length, using at least one reference. All sources must be cited in the text and on the reference page, using APA style.

Unit VII Assignment

Assume that a fatality occurred at your facility one month prior to the OSHA inspection. Review the citations and penalties that were assessed to your facility, and respond to the following questions:

• Which of the citations could be referred to the U.S. DOJ for criminal proceedings?

• What conditions would have to be met before the citations could be referred for criminal proceedings?

• Which individuals working at your facility could face criminal charges under the Act?

• What would be the maximum prison sentence and fines that any individual would face?

• What would be the maximum fine that the company would face?

• If you were facing criminal charges under the Act, what would be your best defense?

• How could you involve the OSHRC in the criminal case(s)?

Your response must be a minimum of two pages in length, using at least one reference. All sources must be cited in the text and on the reference page, using APA style.

Unit 8- Question Answer

1. Summarize the procedures required to achieve Star status under OSHA’s Voluntary Protection Program (VPP).

2. Describe the role that Challenge Administrators play in the OSHA Challenge voluntary cooperative program.

3. Provide your opinion as to which OSHA voluntary cooperative program would be the most beneficial to a small business that prints business cards and letterhead.

4. Discuss the benefits that OSHA alliances provide to employers and workers in general industry.

Identification of Common Minerals

 

Table 1. Identification of Common Minerals

Examine the photograph of each mineral in the textbook (smaller versions are provided here), and note any conspicuous features you see. Identify each mineral by comparing the characteristics provided below with characteristics in the textbook (see Sections 4.3, 4.5, 4.8, and 4.9). If you are interested in viewing the actual mineral specimens, feel free to stop by my office.

image1.jpg Characteristics of this mineral: six-sided crystal, hardness of 7, conchoidal fracture instead of cleavage, partially transparent, does not effervesce with dilute HCl.

Mineral identification:

image2.jpg Characteristics of this mineral: hardness of 3, three directions of cleavage (cleaves into rhombs), partially transparent, effervesces with dilute HCl without being pulverized into a fine powder.

Mineral identification:

image3.jpg Characteristics of this family of minerals: very soft (but not listed on Moh’s Hardness Scale), feels sticky when wet, does not effervesce, can expand when wet. It is not talc or graphite.

Mineral identification:

image4.jpg Characteristics of this mineral: hardness of 2 (can be scratched with a fingernail), cream-colored to partially transparent, does not effervesce.

Mineral identification:

image5.jpg Characteristics of this mineral: sheet-silicate mineral with one dominant direction of cleavage (breaks into flakes and sheets), thin sheets are silvery gray and partially transparent, does not effervesce.

Mineral identification:

image6.jpg Characteristics and identification of these minerals: contain copper and include malachite (green copper-carbonate mineral), azurite (blue copper-carbonate mineral), and native copper (a metallic, copper-colored mineral).
image7.jpg Characteristics of this mineral: can be black, brown, silvery gray, or earthy red, but consistently has a red streak, can have a metallic or earthy luster, is nonmagnetic, and does not effervesce.

Mineral identification:

Table 2. Description of Each Part of House
Below is a description of the function of each part of a house. Use the characteristics of each mineral, and the uses of minerals in Section 4.14, to decide which minerals or materials can be used to build each part. Write the name of the mineral or material in the space provided.
Part of House Function Mineral or Geologic Material Used
Roof A roof is a barrier to rain and snow. Some type of mineral product is used to cover the plywood sheets on the roof.
Insulation To keep the house at a comfortable temperature, a material that slowly conducts heat is placed outside, inside, or within the exterior walls. Commonly, this material is fiberglass, which is produced by melting a common and inexpensive silicate rock and turning the melt into glass fibers.
Exterior walls The outside walls act as a barrier to rain and snow and to support the roof and rest of the structure.
Windows These let in visible light and other solar energy and provide visibility to the outside.
Electrical wiring A material that conducts electricity is used for electrical wiring. Most wire is made from a metal because metals are conductive and ductile (can be shaped easily into wire).
Plumbing Metal pipes are commonly used to carry fresh water into the house and from one part of the house to another.
Inside of walls Interior walls separate the house into rooms, but commonly do not support the structure. They typically have vertical beams (called studs) of a strong material that supports sheets of wallboard that form the actual wall. The interior walls should be soft enough so holes can be cut for electrical outlets and switches.
Cement slab Cement is used to make a fairly smooth, stable base for floor tile, wood, or carpet. It is also used as a foundation to support the walls.
Reference Page (you can print out this page for reference, but you will not turn it in)

The figure below identifies the different parts of a house. In Table 2, write the name of the mineral or material used to build each part of the house. Table 2 describes the function of each part of the house.

image8.jpg

AFTER COMPLETING THIS INVESTIGATION, CLICK ON MOODLE LINK “ASSIGNMENT #2A: MINERALS MULTIPLE CHOICE QUIZ” TO ANSWER 15 MULTIPLE-CHOICE QUESTIONS IN MOODLE. YOU DO NOT NEED TO TURN IN WORKSHEET, JUST ANSWER MULTIPLE CHOICE QUESTIONS WHICH ARE WORTH A TOTAL OF 40 POINTS.

Insulation: ___________

Roof: ______________

Exterior walls: __________

Windows:�__________

Cement: _____________

Electrical wiring:

______________

Inside walls: ___________

Plumbing: ____________

1. In a human population, the genotype frequencies at one locus are 0.75 AA, 0.20 Aa, and 0.05 aa. What is the frequency

1. In a human population, the genotype frequencies at one locus are 0.75 AA, 0.20 Aa, and 0.05 aa. What is the frequency

of the A allele [f(A)] and a allele [f(a)] for the population? Are they in Hardy-Weinberg equilibrium? (5 pts)

2. Calculate the number of heterozygotes in a population with p = 0.6 and q = 0.4 (at time = 0). After 4 generations of

inbreeding between siblings (F = 0.25) in a population of 650. (6 pts)

3. Human albinism is an autosomal recessive trait. Suppose that you find a village in the Andes where 8/1000 of the

population is albino. If the population size was 1600 and the population is in Hardy-Weinberg equilibrium with

respect to this trait, how many individuals are expected to be carriers (heterozygotes)? (5pts)

4. A boatload of 400 Swedish tourists, all of whom bear the MM blood group genotype, are marooned on Haldane Island,

where they are met by an population of Islanders totaling 1000, all bearing blood group NN genotype. In time, the

castaways become integrated into Island society. Assuming random mating, no mutation, no selection (based on

blood group), and no genetic drift, what would you expect the blood group distribution to be among 320 progeny of

the new Haldane Island population? (5 pts)

5. You identify a population of mice (Peromyscus maniculatus) on an island. Their coat color is controlled by a single

gene: BB mice are black, Bb mice are gray, and bb mice are white. You take a census of the population and record

the following numbers of mice:

Black 1156

Gray 408

White 36

(a) What are the frequencies of the two alleles? (4 pts)

(b) What are the Hardy-Weinberg equilibrium frequencies for these three phenotypes? (4 pts)

(c) A heat wave hits the island. All mice with black fur die from heat stroke, but the other mice survive. What are the new

allele frequencies for the population? (4 pts)

(d) If the population suffers no further cataclysms after the heat wave, and the surviving animals mate randomly, what will

be the frequency of mice with black fur in the next generation? (4 pts)

(e) If the climate is altered permanently, so that mice with black fur die before reproducing, which following statement is

correct? (5 pts)

(1) At Hardy-Weinberg equilibrium, f(B) will equal 0.135.

(2) The fitness of mice with gray fur (ωBb) must be equal to 0.5.

(3) The fitness of mice with black fur (ωBB) is 0.

(4) The B allele will disappear from the population in one generation.

(5) The B allele will disappear from the population in two generations.

6. Which of the following are requirements for evolution by natural selection? Explain your answer. (8 pts)

I Environmental change

II Differential survival and reproduction

III Heritability of phenotypic variation

IV Variation in phenotype

V Sexual reproduction

A) II, III, V

B) II, III, IV

C) I, II, IV

D) III, IV, V

E) II, IV, V

7. Which of the following processes is the source of genetic variation within populations? (8 pts)

A) Reproductive Isolation

B) Mutation

C) Selection

D) Asexual reproduction

E) Genetic drift

Explain your answer including a description of the impact of each on genetic variation.

8. If the population (17,377 in 2018) of folks in Perry, GA, had an f(a) = 0.2 and folks in Valdosta, GA, has a f(a) = 0.6,

then how many people from Valdosta, GA, would have to migrate to Perry to increase the population to

approximately f(a) = 0.3? (5 pts, remember, you can’t have fractional people)

9. What is the Ne of a population with the following annual censuses, [note: the drop in size due to 2010 and 2011 being

extreme drought years]? (5 pts)

2008: 9730

2009: 8810

2010: 3653

2011: 420

2012: 94

2013: 1560

2014: 5650

2015: 8700

2016: 9700

2017: 12110

2018: 15060

2019: 30789

10. Consider the following populations that have the genotypes shown in the following table:

Population AA Aa aa

1 1.0 0.0 0.0

2 0.0 1.0 0.0

3 0.25 0.50 0.25

4 0.25 0.25 0.50

5 0.333 0.333 0.333

6 0.0225 0.255 0.7225

7 0.5929 0.3542 0.0529

8 0.9604 0.0392 0.0004

a. What are p and q for each population? (4 pts)

b. Which of the populations are in Hardy-Weinberg equilibrium? (4 pts)

c. Populations 1 and 2 have a tree fall across their islands so that individuals can cross. If equal numbers of the

individuals occur on each island, what is the new population’s allele frequencies and genotype frequencies

after one generation of random mating? (6 pts)

d. In population 3, the a allele is less fit than the A allele, and the A allele is incompletely dominant. The result

is that AA is perfectly fit (= 1.0), Aa has a fitness of 0.8, and aa has a fitness of 0.6. With no mutation or

migration, graph the allele frequency of the a allele after 10 generations under selection (e.g., Time 0 = q

above, Time 1 = first generation after selection) (8 pts)

e. In population 8, the population size gets radically reduced to 50 individuals, total. What is the most likely

fate of the “a” allele, and what genetic principle would lead you to believe that the case? (4 pts)

11. You digest a linear piece of DNA with two restriction enzymes, BamH1 & Sma1, and get the following sized

fragments (in kb [kilobases]) (10 pts):

BamH1 Xho1 BamH1 & Xho1

10 kb 12 kb 8 kb

6 kb 8 kb 6 kb

4 kb 4 kb

2 kb

Draw the appropriate restriction fragment map based on this data labeling all restriction sites

M132 Module 06 Coding Assignment

M132 Module 06 Coding Assignment

1. Case Study #1

PREOPERATIVE DIAGNOSIS: Carcinoma of the right breast, status post neoadjuvant chemotherapy.

POSTOPERATIVE DIAGNOSIS: Carcinoma of the right breast, status post neoadjuvant chemotherapy.

PROCEDURE PERFORMED: Right modified radical mastectomy, left prophylactic mastectomy

PREOPERATIVE HISTORY: The patient is an unfortunate 37-year-old woman who had a pregnancy associated breast cancer of the right breast with extensive involvement of the breast, clinically a stage III breast cancer. She underwent neoadjuvant chemotherapy with a complete clinical response to therapy with no residual palpable tumor in the breast and no palpable adenopathy. She has elected to undergo a bilateral mastectomy. She will have reconstructive surgery at a later time.

OPERATIVE NOTE: The patient was taken to the operating room. General anesthesia was induced. A Foley catheter was inserted. Her arms were placed on pads. Her legs were placed on pads. Bear hugger was applied and her entire upper torso was sterilely prepped and draped in usual fashion. Symmetric skin sparing mastectomies were planned incorporating the nipple-areolar complex on both sides. We began on the left side. An elliptical incision was made incorporating the nipple-areolar complex, carried down through the skin into the subcutaneous tissue. Flaps were raised circumferentially from the superior aspect to the clavicle, medially to the midline, inferiorly to the inframammary, fold and laterally out to the latissimus dorsi. The breast was then removed from the pectoralis major muscle incorporating the fascia, reflected laterally and truncated. It was marked for orientation, weighed and sent to pathology. Hemostasis was achieved where necessary using electrocautery. There was no evidence of bleeding at the end of the case. Moist laps were placed under the flaps and we moved to the right breast. Again, an elliptical incision was created incorporating the nipple-areolar complex and a little more skin laterally in that breast because the breast was a larger breast on that side. Flaps again were raised from superior infraclavicular and a portion of the breast circumferentially to the midline and subsequently to the inframammary fold and subsequently out to the latissimus dorsi muscle. The breast was removed from the pectoralis major muscle incorporating the fascia, reflected laterally. The clavipectoral fascia was opened and a level I and level II axillary lymph node dissection was performed on both sides, sparing the long thoracic and the thoracodorsal neurovascular bundle, as well as at least 1 intercostal brachial cutaneous nerves. The axillary lymph nodes will be examined for metastasis. There was no palpable adenopathy in level III. The breast and axilla were marked for orientation, weighed and sent to pathology. Irrigation was performed. Hemostasis was achieved where necessary using some Surgiclips and electrocautery. There was no evidence of bleeding at the end of the case.

ICD-10-PCS Code: Click here to enter text.

2. Case Study #2

PREOPERATIVE DIAGNOSIS: Open wound left lower extremity status post fasciotomies of the left lower extremity for compartment syndrome status post external fixator for left tibial plateau fracture.

POSTOPERATIVE DIAGNOSIS: Open wound left lower extremity status post fasciotomies of the left lower extremity for compartment syndrome status post external fixator for left tibial plateau fracture.

PROCEDURE PERFORMED: Irrigation and debridement of the left lower extremity down to muscle with primary wound closure of the medial and lateral wounds, both greater than 10 cm each.

ANESTHESIA TYPE: General.

ESTIMATED BLOOD LOSS: Less than 10 mL.

COMPLICATIONS: None.

INDICATIONS FOR SURGERY: The patient is a 59-year-old male with the above diagnosis. The patient had initial application of external fixator and fasciotomies performed by my partner, on November 23rd. The patient had open wounds, initially had application of a wound VAC with the intent to bring him back to the operating room for repeat I and D, possible ORIF, possible wound closure. Preoperatively, the patient’s leg was and he had too much soft tissue swelling. He did not have a positive wrinkle sign so the soft tissues were too swollen to proceed with definitive fixation, so the decision for maintaining the fixator and just doing irrigation and debridement along with possible wound closure was made at that time. Risks and benefits were explained to the patient. He made an informed decision to proceed with the above procedure.

PROCEDURE: The patient seen preoperatively. The left lower extremity was marked. He was brought in the operating room, placed on the operating table, given a general anesthetic. The left lower extremity was then thoroughly prepped and draped in standard orthopedic fashion. Once that was done, universal protocol of a time-out was taken to confirm that the left lower extremity was the correct operative site. Once that was done, 3 liters of lactated Ringer’s laced with bacitracin was used for both medial and lateral wounds. Any nonviable or necrotic tissue was debrided down. Majority of the muscle seemed healthy, was contractile with electrocautery. There was not an excessive amount of bleeding so the wounds were closed primarily. Both medial and lateral wounds with interrupted subcutaneous 2-0 Vicryl for the subcutaneous layer and a running 4-0 V-Loc for the skin. Wounds were then dressed with Steri-Strips, Xeroform, 4 x 4’s and Ace wrap. Xeroform was also placed around the pin sites for the external fixator which was also prepped out from the procedure. The patient was also noted to have some fracture blisters and several abrasions to the skin. Once the leg was dressed, the patient was extubated and

transferred to postanesthesia recovery unit in stable condition. All sponge and sharp counts were correct.

The patient received pre and will receive postoperative antibiotics. He is nonweightbearing. He will be placed back on his anticoagulant treatment most likely Lovenox for DVT prophylaxis and he will be discharged at the discretion of Trauma Service to follow up in the office for reevaluation and determine when definitive fixation will be performed.

ICD-10-PCS code: Click here to enter text.

3. Case Study #3

Do not code the X-ray or fluoroscopic guidance for this case.

PREOPERATIVE DIAGNOSIS:

1. Comminuted right femur fracture secondary to multiple gunshot wounds.

2. Status post multiple gunshot wounds with open wounds, right thigh.

POSTOPERATIVE DIAGNOSIS:

1. Comminuted right femur fracture secondary to multiple gunshot wounds.

2. Status post multiple gunshot wounds with open wounds, right thigh.

PRINCIPAL PROCEDURE PERFORMED:

1. Irrigation/and excisional debridement with primary closure of multiple gunshot wounds, right thigh, encompassing two wounds measuring 2 cm, one wound measuring 3 cm, one wound measuring 4 cm, one wound measuring 6 cm.

2. Open reduction/internal fixation/trochanteric femoral intramedullary nailing, right comminuted femur fracture, with Stryker GTN femoral intramedullary nail.

3. Use of x-ray/fluoroscopic guidance and interpretation.

ANESTHESIA: General.

The patient is a 25-year-old gentleman status post multiple gunshot wounds. He was brought to the Medical Center as a code yellow multi-trauma patient. He was emergently taken to the operating room last night for exploratory laparotomy. At that juncture, his gunshot wounds to his right thigh were irrigated and packed per the trauma service. He has been cleared for surgical stabilization of his comminuted right femur fracture. X-rays have shown him to have a comminuted midshaft femur fracture secondary to his multiple gunshots. At this juncture, it was elected to bring him to the operating room for surgical stabilization of his fracture, irrigation/debridement of his gunshot wounds, with primary closures of the open wounds. Preoperative consent was obtained from the patient’s mother. The patient has been cleared for surgical intervention per the trauma service.

The patient was brought to the operating room from the surgical intensive care unit. He was intubated and sedated. He was transferred onto the fracture table in the supine position. After the establishment of adequate general anesthesia, his right lower extremity underwent an initial irrigation, debridement and closure. The patient was placed on the fracture table and then his right lower extremity was prepped and draped in the usual normal sterile fashion. He did receive preoperative antibiotics. After adequate prepping and draping, his gunshot wounds noted to be five, two of them encompassing approximately 2 cm in length, one measuring 3 cm in length, one measuring 4 cm in length, and the fifth measuring 6 cm in length. All wounds were thoroughly debrided, this encompassing sharp dissection with a scalpel for the skin, subcutaneous tissues muscle and deep tissue. The posterior large wound also had several small bony fragments secondary to the marked comminution of his fracture. These dysvascular fragments with no soft tissue attachment were removed. The wounds were then copiously irrigated with pulsatile lavage. Three liters of pulsatile lavage antibiotic solution were initially irrigated through all the gunshot wounds, followed by an additional 3 liters of normal saline. Status post this, the skin edges were again sharply debrided; the tissue including muscle and subcutaneous tissue were also removed.

The wounds were then closed in layers. The subcutaneous tissues were then reapproximated using 2-0 Vicryl in an interrupted suture ligature fashion. The skin edges were then reapproximated using 2-0 nylon in an interrupted suture ligature fashion. Status post this, the patient was maintained on the fracture table and a gentle reduction of the patient’s comminuted fracture was accomplished, this using the fracture table and C-arm fluoroscopic guidance. Approximate measurements of the patient’s lower extremities were also obtained using the external ruler from the Stryker GTN trochanteric nail system. Measurements were approximately taken of the left femur and the right two approximate limb lengths. Status post, this reduction was maintained and the patient’s right hip and lower extremity were prepped and draped in usual normal sterile fashion. He again did receive preoperative antibiotics.

After adequate prepping and draping, the planned incision was mapped out using C-arm fluoroscopic guidance, this extending from the tip of the trochanter cephalad. The use of x-ray/fluoroscopic guidance was a medical necessity for this procedure, this in an effort to visualize the femur, visualize the reduction and maintain the reduction. The placement of the intramedullary nail necessitated the use of x-ray/fluoroscopic guidance in addition to the locking of the nail. The images were visualized and interpreted by myself. After adequate prepping and draping, the nail insertion wound was taken down clean and sharply through skin and subcutaneous tissues. Dissection down to fascia was accomplished and the fascia incised in line with the skin incision.

It should be noted that after we had the irrigated and debrided the patient’s gunshots with closures, the patient’s right lower extremity was reprepped and draped with new drapes in a sterile fashion. Dissection down to the fascia was accomplished and the fascia then incised in line with the skin incision. Dissection down to the tip of the trochanter was accomplished. A smooth Kirschner wire was initially utilized and the planned insertion point for a trochanteric nail was accomplished, this placed in the tip of the trochanter and verified to be in good position in the AP, lateral and oblique planes. This was then overreamed using a triple reamer. The guidewire was then placed into this and utilizing the fracture reduction tool, the guidewire was manipulated across the fracture region to the distal aspect of the femur. Intraoperative x-rays again revealed good alignment in the AP, lateral and oblique planes. Sequential reaming was then begun using a 9-mm reamer progressing by 1-mm increments through 14 mm. There was noted to be good positioning of the reamer. The appropriate measurements were taken at this juncture, and the definitive Stryker GTN trochanteric femoral nail was opened. It was then placed onto the inserter, the appropriate amount of rotation dialed in. this placed over the guidewire and then impacted into position. Intraoperative x-rays again revealed good alignment in the AP, lateral and oblique planes. Maintenance of reduction was accomplished.

The guidewire was then removed at this juncture. The nail was locked statically, the external alignment jig utilized for the proximal locking screws, one screw placed transversely with the additional screw placed obliquely. Both screws were found to have excellent bite and fixation. They were verified to be within the intramedullary nail. The distal aspect of the nail was then locked. Using the Cole radiolucent drill and the “perfect circle technique,” both locking screws were placed distally in a static mode. Intraoperative x-rays then revealed good alignment in the AP, lateral and oblique planes. Verification that these screws were in the intramedullary nail were accomplished.

All wounds were copiously irrigated with antibiotic solution and suction dried. Hemostasis obtained throughout using Bovie electrocautery. The patient’s deep fascia in the nail insertion was reapproximated using #1 Vicryl in an interrupted suture ligature fashion. All subcutaneous tissues, including the percutaneous screw insertion wounds, were reapproximated using 2-0 Vicryl in an interrupted suture ligature fashion, the skin edges reapproximated using staples. Sterile dressings were placed to all wounds, including the gunshot wounds, with sterile Adaptic gauze, sterile 4×4’s, sterile ABDs, sterile Webril. A Tegaderm was placed on the proximal aspect with Webril and an Ace wrap to the lower extremity as a whole. The patient was transferred back to the surgical intensive care unit in stable condition, having tolerated the procedure well.

Components utilized in this procedure were the Stryker GTN trochanteric femoral intramedullary nail, 13 x 420, with two proximal and two distal locking screws.

Unit 1 Home Lab 1: Metric system

Unit 1 Home Lab 1: Metric system

Points: 40p

 

Name:

 

Purpose (4p)

 

 

 

 

Lab Summary (6p)

 

 

 

 

 

 

Lab Answers (14p)

1. Using a metric ruler, determine the length of the items in Table 1.1 below:

 

In the final column, you are to estimate your measurement precision. To do this, measure each item a second or even third time. How close are the measurements? If there is a range of values for the length you measure, record the average difference between measurement values as your uncertainty. If your measured value for a given object appears the same after repeated measurements, this does not necessarily mean that your uncertainty is zero. Look closely at your ruler or measurement device and estimate the smallest unit of length that you would be able to discriminate with it. Every measurement device has limits. For instance, very few people use a ruler with a precision greater than 1/3 or 1/2 of a millimeter; in many cases, even this precision is difficult or impossible to obtain. Typically +/- 1 mm is standard for measuring flat objects with a ruler, but this uncertainty can be expected to go up when the object has significant curvature or its length is not quite so well defined.

 

To measure the circumference (length around) of your head or thigh, wrap a piece of string around it and mark where the string meets itself. Then lay the string out flat and measure the length with your ruler.

 

Table 1.1. Metric measurements and uncertainties.

meters cm mm inches

 

Uncertainty

?

Your favorite shoe .254 25.4 254 10 2.54
Your index finger 0.0762 7.62 76.2 3 7.62
A pencil 0.1524 15.24 152.4 6 1.524
Fingernail of your pinky 0.003175 0.3175 3.175 1/8 .3175
Width of a credit card
The circumference of

your thigh

The circumference of

your head

2. Measure and record volume in Table 1.2.

 

Estimate the rough volume of your head by using the circumference (denoted C) and multiplying out this formula (based on the volume of a sphere =4pr3/3 = C3/(6p2)):

 

Volume ? 1/59 × C × C × C = C3/59

 

Estimate the uncertainty in your head volume (?V, called “delta V”) calculation by using the uncertainty in your measurement of the circumference of your head (denoted ?C) and multiplying through the following formula:

 

?V ? 3/59 × C × C × ?C = 3/59 × C2 × ?C

 

Table 1.2. Head volume and uncertainty estimates.

Circumference

(C)

Uncertainty in

Circumference

(?C)

Head Volume

 

? 1/59 C3

Uncertainty in

Head Volume (?V)

? 3/59 × C2 × ?C

 

 

3. Complete the conversions in Table 1.3. The first row has been done.

 

Table 1.3. Length conversions.

Length km m miles feet
2.0 km 2.0 2,000 1.24 6,562
705 m 705
3.25 miles 3.25
300 ft 300

 

 

4. Complete the conversions in Table 1.4.

 

Table 1.4. Mass conversions.

Weight kg g pounds (lbs)
5.0 kg 5.0
400 g 400
50 pounds 50

 

 

 

5. Complete the conversions in Table 1.5.

 

Table 1.5. Volume conversions.

Volume liters ml gallons
6.0 liters (l) 6.0
600 ml 600
3 gallons 3

 

 

6. Complete the conversions in Table 1.6.

 

Table 1.6. Temperature conversions.

Temperature °C °F
100 °C 100
27 °C 27
-2 °C -2
27 °F 27
95 °F 95
-40 °F -40

 

 

7. Population biologists use the term “Doubling time” to refer to how long it takes a population to double in size. This concept is particularly useful when the average time for a given individual to reproduce is fairly constant in a species. Consider a bacterial population that can reproduce by dividing into two daughter cells (binary fission) from an original single individual cell. Assume a doubling time of ten minutes and fill out the following table. At time zero there is one bacterium, ten minutes later there are two bacteria, ten minutes after that there are 4 bacteria, etc. Fill in the blanks in Table 1.7.

 

Table 1.7. Population growth.

Number of Bacteria 1 8 First exceeds

10,000

Time 0 30 min 1 hour 2 hour

 

Unit 1 Home Lab 2: Enzymes

Points: 40p

Name:

Purpose (4p)

Lab Summary (6p)

Lab Answers (14p)

1. Fill in the following table. Compare all cups. Use relative terms to describe the size and number of bubbles in each cup. For instance, describe the Number of Bubbles using the terms: No bubbling, Moderate bubbling, Good bubbling, Very good bubbling. To describe average bubble size use the terms: Very small, Small, Large, or Very large. To describe pH without access to pH detectors, simply use the pH chart earlier in this chapter to describe each as acidic, neutral,or basic. To describe the Catalase Activity, use your data on the size and number of bubbles to estimate the amount of gas produced in the Catalase mediated process. Use the following terms: Very Low, Low, Moderate, High, Very high

 

Table 2.1. Catalase reaction observations.

Cup Number of Bubbles Size of

Bubbles

pH Catalase

Activity

1
2
3
4

 

 

2. Bubbling indicates the formation of what chemical?

 

 

3. Describe the activity of Catalase as pH increases. Do you think that other enzymes are likely to behave in this way as well? Why or why not.

 

 

4. Assume that you have a pH meter which would enable you to very accurately measure the pH of a solution. Describe an experimental design that would allow you to pinpoint the exact pH at which Catalase is the most active.

 

 

5. Regarding cup #1:

a) Describe the utility of cup #1 as a control.

b) What other material did you introduce to this cup? Describe what you observed. How does Catalase activity in the material you investigated compare to potato?

Unit 1 Home Lab 3: Cellular Respiration

Points: 40p

Name:

Purpose (4p)

Lab Summary (6p)

Lab Answers (14p)

1. List the following experimental materials:

a) Kind of yeast used:

b) Kind of water used:

c) Average temperature of the water bath during the experiment:

d) Average room temperature during the experiment (estimate if necessary):

e) Duration of yeast solutions exposure to bath:

 

2. List your results in Tables 3.1 – 3.4.

 

Table 3.1. Independent variables and experimental conditions.

Bottle Sugar Yeast Water Yeast solution

height (in cm)

To be heated in warm water bath?
1 1 teasp 2 teasp ¼ cup No. Leave this bottle at room temp.
2 1 teasp 2 teasp ¼ cup Yes.
3 1 teasp 2 teasp ¼ cup Yes. Replicates bottle #2
4 1/3 teasp 2 teasp ¼ cup Yes.
5 No Sugar 2 teasp ¼ cup Yes.
6 ¼ cup

 

Table 3.2. Observations of dependent variables.

Bottle Balloon size Yeast growth Other observations
1
2
3
4
5
6

 

 

 

Table 3.3. Balloon size and solution height measurements.

Bottle Circumference,

C (cm)

Uncertainty in C,

?C

Radius

(long axis, R;

cm)

Uncertainty in R,

?R

 

New height of

yeast solution

(in cm)

1
2
3
4
5
6

 

 

3. In Table 3.4, record yeast growth and estimated volume of each balloon on Bottles 1-6.

 

a. Yeast growth = New height (in Table 3.3) – Original height (in Table 3.1)

 

b. If the balloon did not inflate, it has a volume of zero.

 

c. To estimate the volume of each balloon, use the following formula for the approximate volume of an ellipsoid with a horizontal circumference C and long axis radius R (from Table 3.3):

 

Volume ? 2/19 × (C × C × R)

 

d. To estimate the fractional uncertainty in the volume, use this formula:

 

?V ? 2 × (?C +?R) / C

 

Table 3.4. Yeast growth and balloon volume.

Bottle Independent Variable Yeast growth:

(Change in

solution height)

Balloon Volume

(cm3)

Uncertainty in

Balloon Volume

estimate (?V)

1 No heating
2 Control 1
3 Control 2
4 1/3 teaspoon sugar
5 No sugar
6

 

 

4. Outline the experimental questions in this yeast activity (in a paragraph or two).

 

5. Describe what is measured by the balloon volume. How does it correlate with yeast growth?

 

6. Compare Bottles # 2 & 3. Are they very different? Discuss the utility of having a duplicate measurement when considering the precision of your experimental technique.

 

7. Compare Bottles # 1 to 2 & 3 and discuss the effect of temperature on cellular respiration in yeast.

 

8. Compare Bottles # 2, 3, 4, 5 and discuss the effect of sugar on cellular respiration in yeast.

 

9. Discuss results obtained with your experimental Bottle #6 in comparison with the other experimental conditions.

 

10. In a paragraph or two, describe your conclusions, thoughts about what you learned about cellular respiration, and/or things that went wrong.

Unit 1 Home Lab 4: Genetics and DNA

Points: 40p

Name:

Purpose (4p)

Lab Summary (6p)

Lab Answers (14p)

 

1. Describe what you can see in the final DNA extraction solution. Is the precipitant bubbly or stringy? Does it stick together or does it form many islands?

 

2. List your phenotype for the tongue rolling, ear attachment, and hitch-hiker thumb traits in Table 4.1. Use the following notation:

a) If you can roll your tongue, then your phenotype is R. If you cannot, then your phenotype is r.

b) If your earlobes are unattached, then your phenotype is U. If your earlobes are attached, then your phenotype is u.

c) If you do not have a hitch-hiker thumb, then your phenotype is H. If you do have a hitch-hiker thumb, then your phenotype is h.

 

Use the information above to determine your possible genotypes and record them in Table 4.1. Notice that the phenotype for a given trait is recorded with a single letter, whereas the genotype requires two letters per trait.

 

Then, using what you have figured about your genotype, infer the different possible genotypes that your parents could have had. For instance, if you determine that your possible genotype for earlobe attachment is UU or Uu,then the possible parental genotypes are:

 

Possible parents of UU: UU ×UU; UU ×Uu; Uu × Uu

Possible parents of Uu: UU ×Uu; UU × uu; Uu × Uu; Uu × uu

 

For this question, do not ask your parents about their phenotypes! You will do this in question 3. Question 2 is an exercise in inference based on your understanding of genetics.

 

 

 

 

Table 4.1. Personal phenotype and genotype; inferred possible parental genotypes.

Trait

 

Your

Phenotype

Your possible

Genotypes

Inferred possible parental genotypes

 

 

Tongue rolling

(R or r)

 

Earlobe attachment

(U or u)

 

Hitch-hiker thumb

(H or h)

 

 

 

3. Complete Table 4.2 for you, any blood relatives that you can ask (i.e., parents, siblings, children, etc.), and at least five unrelated “Others” (e.g., spouse, friends, co-workers, etc.). As before, phenotypes for a given trait are recorded with a single letter. You may wish to report separately on your children and spouse in Table 4.3.

 

Table 4.2. Observed parental, sibling, and other’s phenotypes,

Trait

 

Mother’s

Phenotype

Father’s

Phenotype

Relatives’

Phenotype(s)

Others’

Phenotype(s)

 

Tongue rolling

(R or r)

 

Earlobe attachment

(U or u)

 

Hitch-hiker thumb

(H or h)

 

 

In Table 4.2, are there any traits that are particularly common or uncommon among you and your relatives, compared to the unrelated others?